文档介绍：Dtermination of Trace Oxytetracycline in Wasterwater by Phase-
separation Floatation with Ultraviolet Detection
HOU Yan-mina,*, CHENG Yong-huaa, YAN Yong-shengb,
aSchool of chemistry and chemical engineering,pingdingshan university,Henna Pingdingshan,467000,China
bSchool of chemistry and chemical engineering,jiangsu university,Zhenjiang,212013,China
Abstract: In the present work, a novel analytical method was proposed for the determination of trace
oxytetracycline(OTC) in wastewater by phase-separation floatation(PSF) coupled with an ultraviolet detector
setting at 375 nm. OTC can be transformed into plex(Co-OTC-DNA) in the presence of cobalt(II) and
deoxyribonucleic acid (DNA) which can be separated by phase-separation floatation easily. The optimum
phase-separation floatation was performed on phase-separation reagent using 1 mol·L-1 sodium chloride, caustic
soda solution as acidity regulator and tetrahydrofuran(THF) as solvent. The linear regression equation is
A=×105C-, linear range is from ×10-8-×10-5 mol·L-1, lower limit of detection (LOD) was ×10-9
mol·L-1. The recoveries of OTC in water samples were -%.
Keywords: phase-separation floatation(PSF); Ultraviolet detection; oxytetracycline(OTC)
Oxytetracycline(OTC) is widely and gas chromatography with tandem
used in livestock and poultry, aquatic mass (GC-MS