文档介绍：parison of purifying anti-rat TRβ∆
antibodies from the antiserum
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Ying Liu , Bei Sun , Yan Ma , Liling Hu , Xueqin Zhao , Dongchun Liang , Aijun Zuo *,Gang Guo , Jingyu
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Zhang * 1,Institute of Endocrinology and Metabolic Disease Hospital, Tianjin Medical University, 2,Key lab of Hormone
and development, Tianjin Ministry, 3,Dental hospital, Tianjin Medical University, Tianjin 300070, China * Co-Corresponding
to:zhangjingyu2000@ and zuoaijun@
Abstract—The aim of the study was to develop a procedure of
purifying rabbit anti-rat TRβ∆ polyclonal antibody from the II. MATERIALS AND METHODS
antiserum. TRβ∆ was a newly reported thyroid hormone receptor
beta isoform. To gain better understanding of this new receptor, A. Purification of anti-TRβ∆ IgG with protein A
such as its tissue distribution, its specific antibody was needed. In affinity chromatography
this study, chemically synthesized peptide, which is uniquely
existed in the TRβ∆, was used as immunogen to generate antibody For affinity chromatography of IgG, after being settled in the
in the rabbit. Then rabbit anti- TRβ∆ antiserum was purified by column protein A sepharose beads were washed twice with
means of saturated ammonium sulfate precipitation or protein A IgG-binding buffer (10 mM Tris, pH ) to remove sodium
affinity chromatography respectively. pare the e