文档介绍：Study plex Enzyme Based on Extraction of
Total Flavonoids from Bamboo Leaves
Zhang Jian-Wei Zhao Hui, Meng Yan-Qiu
Shenyang Institute of Chemical Technology Shenyang Institute of Chemical Technology
Mechanical Engineering Chemical Engineering
Shenyang, China Shenyang, China
zhangjianwei64@ zhaojingzi521@, mengyanqiu@
Abstract— Use the plant plex enzymes to extract RE-52CS type rotory evaporator (GongYi YingYuYuHua
the total flavonoids from bamboo leaves, comparison with the Instrument Factory); 722 type visible spectrophotometer
traditional ethyl alcohol extraction process. This experiment take (ShangHai JingHua Technology Instrument Factory); DF-101S
the enzyme concentration, the enzymolysis time, the enzymolysis type heat collection-constant temperature type ic stirrer
temperature, the ethyl alcohol concentration, leaching time as the (GongYi YingYuYuHua Instrument Factory)
main check factor, from the single factor analysis and the
orthogonal experiment ensure the best technological conditions of B. Experiment Process
plex enzymes extraction: enzyme concentration is
, enzymolysis time is 60min, enzymolysis temperature is 1) Determination of flavonoid [8]: Rutin as standard on the
55℃, ethyl alcohol density is 40%, leaching time is 150min. Then flavonoids content determination, the maximum absorption wa
through again ethanol