文档介绍：宜春学院学报
Journal of Yichun College
Vol. 33, No. 8
Aug. 2011
• 114 •
第33卷第8期
2011年8月
宜春学院学报
Journal of Yichun College
Vol. 33, No. 8
Aug. 2011
• 114 •
宜春学院学报
Journal of Yichun College
Vol. 33, No. 8
Aug. 2011
• 114 •
碱性蛋白酶高产菌株的筛选
周兰姜
（宜春学院化学与生物工程学院，江西宜春336000）
摘要：实验采用土壤为样品，对样為进行分离，纯化，培养，筛选出了 3株产酶能力较高的苗株，即R1.
R2. R3O复舞确定了 R1的产酹能力相对较高。通过相对酶活力的测定，M定了反应最住温度为30^,最佳pH
为％苗株在最适条件下产酶能力达246. 8U/mlo
关键词：妥白酹；茴掠：筠选；降活力
中图分类号：Q93文猷标志码:A文章编号:1671 -380X (2011) 08 - 0114 - 03
Screening of High Protease Production Strain
ZHOU Lan-jiang
(Chemistry and Biology Engineer cf Yichun university, Yichun 336000 China)
Abstract: Ulis experiment described the separation of strains that could produce protease・ Three strains t designated as R1. R2 and R3 respectively, showed high protease activity was screened from the soil then the second round screening was performed and R1 was determined to have the highest activity t relatively・ By determining the relative enzyme activity 9 the optimal temperature and pH was 30弋 and 9, respectively. The enzyme activity that the strain produced was up to 246・ 8U/mL
Key words： protease； strain； screen； enzyme activity
宜春学院学报
Journal of Yichun College
Vol. 33, No. 8
Aug. 2011
• 114 •
宜春学院学报
Journal of Yichun College
Vol. 33, No. 8
Aug. 2011
• 114 •
蛋白酹是在一定的pH和温度条件下，通过内切和外切作用使蛋白质水解为小肽和氨基酸的生物酹⑴。它在洗涤剂、制革脱毛、