文档介绍：实验2 高效液相色谱法测定栀子药材中栀子苷的含量
实验2 高效液相色谱法测定栀子药材中栀子苷的含量
学时分配：6学时
实验目的和要求
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2by the mobile phase, the components are separated on the stationary phase and each component passes through the detector in succession and thus a chromatogram is record.
The stationary phase and the component part of the mobile phase should be in accordance with the qualities of test samples. Silica gel and chemically bonded silica gel are commonly used as the bulking agent. For chemically bonded silica gel, octadecylsilane type is most frequently used, followed by the octylsilane type, and the cyano and amino group bonded silica gel are occasionally used. The column is maintained at room temperature. The type of detector includes UV, DAD, SPD and so on.
There are two important factors responsible for chromatographic separation efficiency. One is the thermodynamic factor, such as the capacity factor of different components (k’), the other is the dynamic factor, such as the width of peak. The indexes to reflect these physical processes are retention time (t R) and the number of theoretical plates of column (n). Calculate the value of k’ and n as follows:
k’ = (t R－t o) / t o; n = (t R / w1/2)2
The separation of different components will be successful if close attention is paid and great effort is taken to solve the contradiction c