文档介绍：第二代测序数据分析原理详解演示文稿
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优选第二代测序数据分析原理
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三代DNA测序技术之比较
第一代测序技术：Sanger测序法
第二代测序技术：4 RNA-seq
(ChromatinImmunoprecipitation，ChIP)
Meth-seq
ABI SOLiD Re-sequencing
ChIP-seq
RNA-seq
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Experiments
DNA-seq: de novo, resequencing
RNA-seq:mRNA, ncRNA, smRNA...
ChIP-seq: Chromatin ImmunoPrecipitation
Methyl-seq: methylated DNA (epigenome)
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主要的测序平台
基因组分析原理
转录组分析原理
分析策略的选择
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Sequencing Glossary
Reads. A collection of clones that over-sample the target genome.
Pair-end reads derived from both ends of a sequencing-library clone.
Mate-pair reads derived from both ends of a mate-pair library clone which insert size is usually>1kb.
Insert size. The size of the clone-insert from which a clone-end pair is taken.
Contig. The result of joining an overlapping collection of sequence reads.
Scaffold. The result of connectiing non-overlapping contiges by using pir-end reads.
N50 size. As applied to contigs or scaffolds, that size above which 50% od the assembled
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全基因组de nove分析工具
Platform
Correction
Assembly
Solexa
SOAPdenovo
SOAPdenovo Velvet,Abyss
Solid
SAET
Velvet
454
newbler
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分析所需工具
Bowtie software
-http://bowtie-/
SAM tools
-/
TopHat softare
-/
Cufflinks software
-/
CummeRbund software
-/
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外显子组分析工具
Platform
Alignment
Find Variations
Solexa
SOAP,bwa
SOAPsnp samtools
Solid
Bioscope,BFAST
Bioscope,BFAST
454
BLAST,NEWBLER
newbler
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主要的测序平台
基因组分析原理
转录组分析原理
分析策略的选择
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常规分析
Transc