口腔扁平苔藓中Caspase.doc

文档介绍：1 口腔扁平苔藓中 Caspase 作者:姚希,沈丽佳,殷操,谢思明【关键词】扁平苔癣 Expressions of Caspase3, TNF α and TNFR Ι and thEir relationship with cell apoptosis in oral lichen planus 【 Abstract 】 AIM: To examine the expressions and distribution of Caspase3, TNF α and TNFR Ι and thEIr relationship to apoptosis in oral lichen planus (OLP). METHODS: Immunohistochemical staining with SP method and TUNEL technique were used to detect the expressions of Caspase3, TNF α and TNFR Ι in 33 cases of OLP and 7 cases of normal oral mucosa specimens (the controls). RESULTS: Compared with the controls, OLP was increased in apoptosis index (AI) of epithelial cells and decreased in AI of lymphocytes (both ). The expression of Caspase3 in all OLP cases was significantly higher than that in the normal group both in the epithelial layer and in the lamina propria (). pared with that in the normal group, TNF α expression in all OLP cases was decreased in the epithelial layer and increased in the lamina propria (both ), while TNFR Ι expression was increased in the epithelial layer and decreased in the lamina propria in all OLP cases (both ). TNF α expression was significantly increased in the lamina propria in the erosion pared with the nonerosion group (). A positive correlation was observed between Caspase3 expression and AI in the epithelial cells (r=, ). However , there was no significant correlation between Caspase3 expression and AI in lymphocytes (). Expressions of TNF α and TNFRI were significantly correlated to AI both in the epithelial layer and in the lamina propria. CONCLUSION: The accelerated apoptosis of keratinocytes and the inhibition of lymphocyte apoptosis may coexist in OLP, which may contribute to the formation and progression of OLP. 【 Keywords 】 lichen planus, oral; Caspase3; tumor necrosis factor; TNFR Ι; apoptosis 【摘要】目的: 探讨口腔扁平苔藓( OLP )中 Caspase3, TNF α和 TNFR Ι的表达意义及其与细胞凋亡的关系. 方法: 3 采用脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法( TUNEL 法)、 SP 免疫组化方法检测 33例 OLP 及7 例正常口腔黏膜( 对