文档介绍：·论著·
巴戟天寡糖的高效薄层色谱指纹图谱研究
廖慧君,赖正权,仰铁锤,丁平* ,
广州中医药大学中药学院广州 510405
摘要目的建立巴戟天寡糖的高效薄层色谱 HPTLC 指纹图谱,并对不同产地巴戟天药材、炮制饮片、混伪品及含巴戟天药
材的复方制剂———口服液 X 中寡糖成分进行分析比较。方法在湿度 40% ~ 55% 、温度 20 ~ 32 ℃条件下,以乙酸乙酯-甲醇-
, , ,
水-冰醋酸为展开剂二次展开展距 13 cm α-萘酚试液加热至显色于可见光下检视。将巴戟天寡糖 HPTLC 指纹图谱导入指
纹图谱系统解决方案软件 CHROMAP 1. 5 生成共有模式。结果巴戟天寡糖指纹图谱由 11 个特征条带色谱峰构成,并
指认了其中的果糖、蔗糖、1-蔗果三糖、耐斯糖及 1F -果呋喃糖基耐斯糖 5 个峰。结论通过 HPTLC 指纹图谱考察,炮制后巴
戟天寡糖成分出现质与量的改变来源于 4 省的巴戟天药材无明显差异混伪品中不含巴戟天寡糖不能作为巴戟天替代药材
入药含巴戟天药材的制剂仍可采用该 HPTLC 法进行制剂质量评价与控制。
关键词巴戟天寡糖高效薄层色谱

中图分类号 R284 文献标志码 A 文章编号 1001 - 2494 2011 18 - 1385 - 04
High-Performance Thin-layer Chromatographic Fingerprint Analysis of Morinda officinalis harides
, , , * ,
LIAO Hui-jun LAI Zheng-quan YANG Tie-chui DING Ping College of Chinese Materia Medica Guangzhou University
, ,
of Chinese Medicine Guangzhou 510405 China
,
ABSTRACT OBJECTIVE To establish the HPTLC fingerprint of Morinda officinalis harides and to study the characteris-
,
tic fingerprints of Morinda officinalis How from different producing areas and processed by different methods and its adulterants and to
identify Morinda officinalis How in preparations. METHODS HPTLC were carried out after twice development with ethyl acetate-
methanol-water-glacial acetic acid as developing solvent system and α-naphthol test solution as derivatization reagent. Relative humidity
and ambient tempera