文档介绍：11 1
应用与环境生物学报 2010,16 ( 1 ): 118~121 2010-02-25
Chin J Appl Environ Biol=ISSN 1006-687X DOI: .
甲苯降解菌JB-1的分离、鉴定与降解特性*
冷玲张迹李顺鹏**
(南京农业大学生命科学学院农业部农业环境微生物工程重点开放实验室南京 210095)
摘要从南京石化废水处理池的活性污泥中分离到1株能以甲苯为唯一碳源生长的细菌,命名为JB-1. 根据其生理生
化特征和16S rDNA(GenBank Accession No. EU869278)序列相似性分析,将该菌株鉴定为假单胞菌属(Pseudomonas
sp.). 该菌株在18 mg/L的甲苯. 降解甲苯的最适温度为30 ℃,,降解速率与初始接种量呈
正相关. 甲苯降解途径是通过甲苯双加氧酶形成顺甲苯二氢二醇,再经邻苯二酚2,3双加氧酶开环降解. 图2 参22
关键词甲苯;生物降解;假单胞菌属;甲苯双加氧酶;邻苯二酚2,3双加氧酶;代谢途径
CLC X172
Isolation, Identification and Characteristics of a Toluene-degrading
Bacterium JB-1*
LENG Ling, ZHANG Ji & LI Shunpeng**
(Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University,
Nanjing 210095, China)
Abstract A bacterial strain JB-1 capable o utilizing toluene as sole carbon source was isolated romrom a active sludge collected
from Nanjing Petro-chemical Co., Ltd. It was preliminarily identified as Pseudomonas sp. according to its physiological and
biochemical properties and the similarity analysis o its 16S rRNA gene sequence (GenBank Accession No. EF869278). Strain
JB-1 could degrade mg/L toluene within 18 h. The optimal pH and temperature or the degradation were and 30 ℃,
respectively. The degrading rate showed a positive