喷雾干燥法制备神经毒素壳聚糖鼻腔给药微球的研究.doc

文档介绍：喷雾干燥法制备神经毒素壳聚糖鼻腔给药微球的研究
蔡鑫君,夏爱晓,柴国宝,魏颖慧,冯健,李范珠基金项目:国家自然科学基金资助项目(30371781),国家自然科学基金资助项目(30772793),
第一作者简介:蔡鑫君(1985-),男,硕士研究生,主要研究方向为中药新剂型。Tel:[phone] E-mail:zjtcmcxj@ *通讯作者:李范珠,男,教授,博士生导师研究方向:药物新技术与新剂型研究。Tel:[phone] E-mail:Lifanzhu@
(浙江中医药大学药学院,浙江杭州 310053)
摘要: 目的制备神经毒素壳聚糖微球,并对其体外释药模式进行研究。方法采用喷雾干燥法制备神经毒素壳聚糖微球,以包封产率为指标,考察了处方与工艺因素对壳聚糖微球的影响,并采用正交设计L9(3)4对处方和工艺进行了优化。%,壳聚糖-TPP重量比为10∶1,进口温度105℃,空气流速550L/h。优化处方制备的神经毒素壳聚糖微球表面圆整,载药量为(±)%,包封产率为(±)%,平均粒径为(±)μm。体外释药数据用Higuchi模型拟合得方程Q=-(r=),表明该微球具有缓释作用。结论采用喷雾干燥法制备微球,具有工艺稳定、可连续操作等优点,制备的神经毒素壳聚糖微球具有较好的缓释特性,
关键词:喷雾干燥法;神经毒素;壳聚糖微球;体外释放
Preparation of Chitosan Microspheres Loaded with Neurotoxin-Ⅰfor Nasal Delivery by Spray Drying Technique
CAI Xin-jun, XIA Ai-xiao, CHAI Guo-bao,WEI Ying-hui, Fen Jian,LI Fan-zhu*
(College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou 310053)
ABSTRACT: OBJECTIVE To prepare the preparation of neurotoxin-Ⅰloaded chitosan microspheres and to investigate the drug release behavior in vitro. Methods Microspheres were prepared using the spray drying method,the encapsulation efficiency was used to evaluate the influence of different formulation and preparation factors,the formulation was optimized by orthogonal design L9(3)4. Results The optimal formulation was as follows:chitosan concentration (%),ratio of chi