文档介绍：2型糖尿病肾损伤大鼠模型的建立和肾脏CD34表达
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作者:胡波,李锋,李敏,王胜春
【摘要】目的观察高脂喂养联合低剂量链脲佐菌素(STZ)注射的SD大鼠2型糖尿病肾损伤模型的代谢特征及CD34的变化。方法 4周龄雄性SD大鼠60只随机分为2组。正常对照组10只,普通饲料喂养;糖尿病模型组50只,高脂饲料喂养,即普通饲料中加入1O%猪油、10%葡萄糖、1%胆固醇、2%蛋黄粉、1%胆酸钠。喂养4周后尾静脉注射STZ(35 mg/kg)。所有大鼠在STZ注射10 d与30 d时做灌胃葡萄糖耐量试验(OGTT)。放免法测定血清胰岛素,免疫组化染色观察肾脏CD34的阳性表达。结果糖尿病肾损伤大鼠空腹血糖(FBG)显著高于正常对照组(),血糖值在注射完STZ后1~3个月比较稳定;24 h尿蛋白从1个月后一直持续上升;空腹血清三酰甘油(TG)和游离脂肪酸(FFA)水平显著高于正常对照组();尿素氮(BUN)、血肌酐(Cr)显著高于正常对照组();在糖尿病肾损伤大鼠的肾脏中CD34出现高表达。结论高脂喂养联合低剂量STZ注射的糖尿病大鼠模型拟2型糖尿病肾病发生的主要病理生理过程,具有高血糖以及血脂异常和肾功能损伤等基本特征;肾损伤可能与CD34有关。
【关键词】糖尿病;大鼠;链脲佐菌素;CD34;动物模型
Abstract:Objective To investigate the metabolism characteristics and CD34 variance of SD rats models with type 2 diabetes renal injury with high grease feeding and low-dose STZ injection. Methods 60 male SD rats with 4-week old were randomized into two groups:10 in the control group feeding with normal forage, 50 in the DM group feeding with high grease forage, which consist of 10% lard, 10% glucose, 1% cholesterol, 2% yolk powder, and 1% sodium cholate adding into the normal feeds. Intravenous injection of STZ after feeding 4 weeks (35 mg/kg). All the rats were tested with OGTT after 10 days and 30 days injection of STZ. Serum insulin was measured by radioimmunoassay (RIA) and immunohistochemistry staining was adopted to observe the positive expression of renal CD34