文档介绍：华中科技大学
硕士学位论文
大鼠乙型肝炎病毒相关性肾炎模型的建立
姓名:李晴
申请学位级别:硕士
专业:内科学
指导教师:周朝阳
2011-05-06
华中科技大学硕士学位论文
大鼠乙型肝炎病毒相关性肾炎模型的建立
摘要
【目的】
建立大鼠乙型肝炎病毒相关性肾炎模型,以探讨建立乙型肝炎病毒相关性肾炎动
物模型的方法,以探讨乙型肝炎病毒相关性肾炎的发病机制。
【方法】
选 SD 雄性大鼠 20 只,200g 左右,适应性喂养一周后分为模型组与对照组,各
10 只;模型组大鼠尾静脉注射 HBsAg、HBeAg 阳性血清,每只 ,2 次/周;
共四周。对照组大鼠仅给予等量生理盐水。造模型前后均检测大鼠尿蛋白等临床表现
及指标,每周一次。蛋白尿阳性时说明大鼠罹患肾炎,一周后杀两组大鼠,取其肾脏,
分别做常规光镜、免疫荧光、免疫组化及电镜检查。
【结果】
模型组大鼠蛋白尿均为阳性,肾脏病理切片显示,光镜下系膜细胞增生、炎细胞
浸润及蛋白管型;PASM-Masson 染色显示:免疫复合物沉积于系膜区、基底膜、内皮
下及上皮下,基底膜不均匀增厚;免疫荧光染色显示 IgM(+~++)、IgG(++~+++),
沉积于系膜区及基底膜;免疫组化检测 HBsAg 沉积于肾小球基底膜,外周襻为主;电
镜下可见肾小球基底膜不均匀增厚,免疫复合物沉积于系膜区、内皮下及上皮下、基
底膜。对照组大鼠蛋白尿均为阴性,普通光学显微镜、荧光显微镜、免疫组化及电镜
检测其肾脏组织均为阴性。
【结论】
通过尾静脉给予 SD 大鼠 HBsAg、HBeAg 阳性血清,模拟 HBV 体内复制过程,可以
建立 HBV-GN 动物模型;HBV 相关抗原抗体复合物沉积是乙型肝炎病毒相关性肾炎的发
病机理之一;病理类型以不典型膜性肾病为主。
【关键词】乙型肝炎病毒相关性肾炎动物模型免疫复合物

I
华中科技大学硕士学位论文
The Establishment of the model of rats of Hepatitis B
Virus-Associated Glomerulonephritis
Abstract
【 Objective 】 To establish the model of rats of Hepatitis B Virus-Associated
Glomerulonephritis, and to research the method of establishing animal model of Hepatitis B
Virus-Associated Glomerulonephritis, and to analyze the pathological mechanism.
【Methods】20 male SD rats, weight 200g, received food and water. After one week, then
they were divided into two groups randomly: the normal group, model group. Model rats
were induced by injection of HBsAg, HBeAg positive serum via tail veins, ,
twice a week for 4 weeks. The rats of health control group were given injection of
physiological saline, in the same way. Observation indices included main symptoms and
signs, urinary protein. Before and after building models, they are measured weekly. When
the model rats had proteinuria, they were diagnosed nephrotitis, and after one week, we
dissected two groups, and took their kidney tissue, and observed them at Light Microscopic,
Immunoflu-orescent microscopy, immunohistochemical, and electron m