文档介绍：肠缺血再灌注损伤大鼠肠黏膜组织中TNFα和VIP的表达及意义
【摘要】目的探讨肠缺血再灌注损伤大鼠肠黏膜组织中肿瘤坏死因子α(TNFα)和血管活性肠肽(VIP)的表达及意义。方法健康Wistar大白鼠60只,随机分为实验组、对照组和正常组,每组20只。实验组通过手术用无损伤动脉夹夹闭肠系膜上动脉起始部,1 h后松夹恢复肠系膜上动脉血流制成肠缺血后再灌流模型;对照组只进行同样腹部手术操作但不夹闭肠系膜上动脉;正常组不手术。6 h后处死动物,取距回盲部15 cm处肠管1 cm,常规组织切片,通过苏木精伊红染色观察肠黏膜组织细胞学形态;采用免疫组织化学方法染色显示肠黏膜组织或细胞中肿瘤坏死因子α(TNFα) 和血管活性肠肽(VIP)的表达情况,使用VIDAS图像分析系统进行半定量分析。结果光镜下,实验组与对照组相比肠壁变薄,上皮细胞水肿明显,中性粒细胞及淋巴细胞浸润,实验组肠黏膜上皮细胞胞浆及基质TNFα表达较其他组增高(F=,P&lt;);肠黏膜上皮细胞内血管活性肠肽表达较其他组增高(F=, P&lt;)。结论 TNFα和VIP表达升高与肠损伤有关,纠正TNFα和VIP的平衡,可对临床预防和治疗肠损伤的发生提供新的方法。
【关键词】再灌注损伤肠屏障肿瘤坏死因子α血管活性肠肽
[ABSTRACT]ObjectiveTo explore the expression and its significance of tumor necrosis factor alpha (TNFα) and vasoactive intestinal peptide (VIP) in impaired intestinal
Sixty healthy Wistar rats were evenly randomized to study, control and normal group. For study group, a model of postischemicreperfusion intestine was established by clamping the superior mesenteric artery; for control group, a laparotomy was merely done; for those in normal control group, no operation was done. An 1 cm long segment of intestine, 15 cm above ileocecal junction, was dissected 6 hours later, and a tissue section made. Immunohistochemical study was used to observe the expression of TNFα and VIP and VIDAS image analysis system applied for semiquantitative enteric wall, edematous epithelium with ne