文档介绍：葡萄球菌生物膜检测及与耐药性的关系
作者:张丽萍,李清,古丽娜依明,李玲玲,田刚
【摘要】目的探讨临床标本中葡萄球菌形成生物膜的状况,研究有无生物膜的葡萄球菌的耐药性差异,帮助临床根据药敏结果选用抗菌药物,及时有效控制葡萄球菌医院感染。方法采用刚果红,微量平板法检测生物膜形成,观察葡萄糖、乙醇、氯化钠对生物膜形成的影响;对临床标本分离的葡萄球菌进行鉴定和药敏试验。结果刚果红法检测腐生葡萄球菌、金黄色葡萄球菌(金葡菌)﹑%、%、%;生物膜半定量法:表皮葡萄球菌生物膜阳性率TSB、TSB%Glu、TSB2%Alc、TSB4%Alc、TSB2%NaCl、TSB4%%、%、%、%、%、%,%Glu、TSB2%Alc、TSB4%NaCl为最佳浓度;药敏结果,产生生物膜的葡萄球菌的耐药性对青霉素、红霉素和复方新诺明的耐药有增加趋势,耐药率分别为:%、%、%。结论临床标本葡萄球菌绝大部分能产生生物膜、葡萄糖、乙醇、氯化钠能促进生物膜形成;葡萄球菌对临床常用的抗菌药物耐药性严重,对其引起的感染可用万古霉素。
【关键词】葡萄球菌生物膜耐药性药敏试验

【Abstract】 Objective To study the formation of al biofilm and the difference of drug resistance between us with or without biofilm,h
elping to choose the antibiotic according to the drug sensitive trial,controlling the al inffectin effectivly and Biofilmforming ability was determined by congo red agar (CAR) and a semiquantitative microtiter assay using trypticase soy broth .Glucose,alcohol and Nacl were added to the medium separately to test their effect on biofilm formation by al Biofilm formation was positive by % to us saprophyticus,% to us Aureus, 40% to us epidermidis by congo red agar. The positive rate of biofilmforming by us epidermidis in TSB, TSB% glucose, TSB2% alcohol, TSB4% alcohol, TSB2% NaCl and TSB4% NaCl were %、%、%、%、% and 32.