文档介绍：TRAIL联合PDTC对SGC-7901细胞生长抑制和凋亡诱导的实验研究
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【摘要】目的观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)及TRAIL联合核转录因子核因子-κB(NF-κB)活性特异性抑制剂吡咯烷二硫代氨基甲酸盐(PDTC)对胃癌细胞SGC-7901的生长抑制和凋亡诱导作用,并寻找逆转TRAIL耐药的方法。方法采用不同浓度的TRAIL及TRAIL联合PDTC作用于胃癌细胞后,MTT法检测细胞生长抑制率,流式细胞仪检测细胞的凋亡率,免疫细胞化学染色方法观察药物作用前后NF-κB表达情况。结果单独使用TRAIL时,随着浓度从50 μg/L增加到500 μg/L,细胞的生长抑制率和凋亡率逐渐增加(),但这种作用是非线性的。 μmol/L、 μmol/L PDTC后肿瘤细胞的生长抑制率和凋亡率较单用TRAIL显著下降();而联合1 μmol/L、10 μmol/L PDTC后其作用较上有所上升()。联合作用组 p65蛋白在胞核中染色的强度低于单纯使用TRAIL组()。结论 TRAIL对胃癌细胞株SGC-7901具有一定程度的生长抑制和凋亡诱导作用;NF-κB信号转导途径可能具有双向调节作用,大剂量PDTC可能逆转胃癌细胞对TRAIL的耐药。
【关键词】肿瘤坏死因子相关凋亡诱导配体;吡咯烷二硫代氨基甲酸盐;胃癌细胞;凋亡;核因子-
κB
Abstract:Objective To observe the effect of bination of pyrrolidine dithiocarbonate (PDTC), a specific inhibitor of NF-κB and tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) on the apoptosis of gastric cancer cells and their effect on the expression of NF-κB, and to search for the approaches to reversing resistance to TRAIL in human stomach cancer cells. Methods Various concentrations of TRAIL and PDTC were added in exponential growth SGC-7901 gastric cancer cells. MTT assays were used to measure the growth inhibitory effect bined use of TRAIL and PDTC or alone. Apoptosis of gastric cancer cell SGC-7901 was analyzed with PI staining method and flow cytometry (FCM). The expression of NF-κB was observed using immunohistochemical staining method. Results After the SGC-7901 gastric cancer cells were exposed to TRAIL at a dose of 50 μg/L to 500 μg/L, the growth inhibitory rate and apoptosis rate increased (P ), but there was no linearity for this dose-effect relationship. Following TRAIL treatment bination with
μmol/L and μmol/L PDTC, the growth inhibitory rate of and apoptosis rate significantly pared with treatment with TRAIL or PDTC alone (), while following TRAIL treatment bination with PDTC 1 μmol/L and 10 μmol/L, the growth inhibitory rate and apoptosis rates pared with