文档介绍：β3GalT7基因转染HL60细胞及对其生物学行为的影响
(作者:___________单位: ___________邮编: ___________)
作者:行书丽,岳爱环,唐春花,贾伟,吴士良]
【摘要】目的: 初步探讨人类β1,3半乳糖基转移酶7(β3GalT7)基因对HL60细胞的细胞周期、侵袭能力等方面的影响。方法: 通过脂质体介导将本实验室构建好的β3GalT7真核正义表达载体pEGFPC1T7Sense和反义表达载体pEGFPC1T7AntiSense转染入HL60细胞;流式细胞仪分析各细胞株的细胞周期改变;Transwell侵袭实验检测β3GalT7对HL60细胞侵袭转移能力的影响。结果: 随着β3GalT7 表达的增高,G2+S期细胞增多,且细胞侵袭能力增强;而转染反义表达载体pEGFPC1T7AntiSense的结果是相反的。结论: 过度表达β3GalT7的HL60细胞株体外增殖和侵袭能力增强。
【关键词】β1,3半乳糖基转移酶7; 正义表达载体; 反义表达载体; 细胞周期; 侵袭
[Abstract]Objective: To explore the effects of β3GalT7 gene on cell cycle, invasive potential of HL
: Transfect the binant plasmids pEGFPC1T7Sense and pEGFPC1T7AntiSense into HL60 with cycle and invasive potential of the transfected cells with flow cytometry and transwell plate,respectively. Results: As the upregulation expression of β3GalT7, the cell growth rate increased. At 48 h after transfection, significantly increased invasiveness was noted in HL60 transfected with pEGFPC1T7Sense. Conclusion: Overexpressing β3GalT7 in HL60 enhanced proliferative and invasive potential.
[Key words]beta 1, 3galactosyltransferase 7;plus sense binant plasmid; antisense binant plasmid;cell cycle;invasive potential
β1,3半乳糖基转移酶家族是糖基转移酶超家族中最具代表性的酶家族之一,β1,3半乳糖基转移酶7(beta1,3galactosyltransferase 7,β3GalT7)基因是我们实验室从人肺cDNA文库中克隆到的人类β3半乳糖基转移酶家族中的一个新成员[1,2]。

在此之前,β1,3半乳糖基转移酶家族已经克隆到6个