文档介绍：百合多糖的分离纯化及抗肿瘤作用
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作者:弥曼,李汾,任利君,梅其炳
【摘要】目的从百合粗多糖中分离纯化出纯化的百合多糖,并观察其抗肿瘤作用。方法用水提醇沉法从植物百合中提取百合粗多糖,经透析、除蛋白、冷冻干燥后,采用DEAE阴离子交换纤维素分段洗脱对其进行分离纯化,得到纯化的百合多糖;将纯化的百合多糖用于H22荷瘤小鼠,测定其对荷瘤小鼠免疫功能的影响及抗肿瘤作用。结果分离出了纯化的百合多糖,经纯度鉴定表明成分均一;该多糖能增强H22荷瘤小鼠的免疫功能,抑制H22肿瘤的生长。结论纯化的百合多糖成分均一,具有抗肿瘤作用。
【关键词】百合多糖;分离纯化;抗肿瘤作用
ABSTRACT: Objective To isolate and purify crude lily haride and observe antitumor activity of the isolated and purified haride. Methods Crude lily haride was extracted from lily by water extraction and ethanol precipitation. After dialyzing, deproteining and freezedrying, preliminary purification of crude lily haride was obtained. After being separated by anion
exchange chromatography, preliminary purification was further isolated and purified. Purified lily haride was given to the H22bearing mice. The effects of purified lily haride on tumor weight and immune function were evaluated. Results Crude lily haride was isolated and purified essfully. After a Sephadex chromatography, the purified haride showed as a single peak, demonstrating its homogenicity. The purified lily haride showed an inhibitory effect on H22bearing mice tumor growth. It significantly increased the weight of ans and improved the immune function of the mice. Conclusion The purified lily haride was homogeneous. It could inhibit H22 tumor growth and enhance nonspecific immune functions in H22bearing mice. This research has laid the foundation for further pharmacological study on lily haride.
KEY WORDS: lily haride; isolation and purification; antitumor activity
百合中主要含有酚酸甘油酯、苷类、生物碱及多糖,另外还含有一些磷脂、蛋白质和无机元素。对百合保健功能的研究已开始从单纯药理研究向保健功能成分的研究转变。国外已从百合植物碱中分离得到秋水仙碱,并对其抗癌作用做了研究。另有研究表明,从百合中提取的皂苷具有止咳、平喘等功能,而作为百合中主要功能成分之一的百合多糖的组成却不是很明确。本研究从百合粗多糖中分离出百合中性多糖、百合弱酸性多糖、酸性多糖及蛋白质,并对中性多糖进行了抗肿瘤及非特异性免疫调节研究。
1 材料与方法
主要试剂与仪器百合粗多糖干燥制剂(自制);DEAE
纤维素(上海生化试剂公司);SephadexG200(上海生物工程公司);其他试剂均为国产分析纯。大容量离心机、旋转蒸发器、烘箱、透析袋(截留分子质量8000,华美生物工程公司);磁力搅拌器、HL2B cm数显恒流泵、梯度混合仪、SBS100自动分部收集器(上