文档介绍：白细胞介素6对HL-60细胞NF-κB p65活性的影响
【关键词】白细胞介素
摘要:目的研究白细胞介素6(interleukin-6,IL-6)对粒细胞白血病细胞系HL-60核因子-κB p65(NF-κB p65)活性的影响,以探讨IL-6在白血病发生、发展中的作用。方法指数生长期HL-60细胞置于96孔培养板内RPMI-1640培养液培养2h后,分为A、B2组。A组加PBS液继续培养3h;、5、-6继续培养3h。用流式细胞仪(FCM)检测HL-60细胞NF-κB p65的活化率。结果 IL-6浓度为5、-60细胞NF-κB p65活化率分别为(±)%、(±)%,与对照组(±)%相比差异有统计学意义(P<);IL--κB p65活化率为(±)%,与对照组相比差异无统计学意义(P>);3种IL-6浓度组间的NF-κB p65活化率比较差异有统计学意义(P<)。结论 IL-6有诱导HL-60细胞NF-κB p65活化的作用,且存在剂量依赖趋势。
关键词:白细胞介素6;HL-60细胞;核因子-κB p65;活化
Abstract:Objective To explore the effects of interleukin-6(IL-6)on the activity of nuclear factor kappa B p65(NF-
κB p65)in HL- After being cultured in RPMI-1640for2h,the HL-60cells were divided into A and B culture was continued for3h,with PBS being added in the media of group A,and IL-, added in the media of the respective subgroups of group activation of NF-κb p65of HL-60cells was deter-mined by flow cytometry(FCM).Results The rates of activation of NF-κB p65in HL-60cells cultured with IL- were(±)%and(±)%,respectively,higher than the control(±)%(P<).The activation was(±)%in the case of IL-,different from the control insignificantly(P>).Conclusion IL-6increases the activation of NF-κB p65of HL-60cell