文档介绍：科素亚对心力衰竭大鼠心肌细胞凋亡及Bax、Bcl-2蛋白表达的影响
【摘要】目的应用科素亚治疗慢性压力负荷性心力衰竭大鼠,探讨其对心肌细胞凋亡及Bax、Bcl-2蛋白表达水平的影响,为心力衰竭发病机制及治疗提供实验依据。方法 30只SD大鼠随机分为心衰组(n=10)、治疗组(n=10)和对照组(n=10),采用鼠腹主动脉缩窄术复制心力衰竭模型。治疗组给予科素亚30 mg·kg-1·d-1 灌胃,心衰组和对照组分别给予等体积生理盐水灌胃;3个月后测量血流动力学指标,原位脱氧核糖核酸酶末端标记法(TUNEL)检测心肌细胞凋亡,免疫组织化学染色法检测心肌Bax、Bcl-2蛋白表达。结果心衰组与对照组相比,心功能明显减退(P<),心肌细胞凋亡指数明显增加(P<),心肌细胞中Bcl-2蛋白表达减少、Bax蛋白表达增加、Bcl-2/Bax比值降低;治疗组与心衰组相比,心室重构改善(P<),心肌细胞凋亡减少(P<),心肌细胞中Bcl-2表达增加、Bax表达减少(P<)。结论心力衰竭大鼠心肌细胞凋亡增加与Bcl-2蛋白表达降低、Bax蛋白表达增加有关。减少Bax蛋白表达,同时增加Bcl-2蛋白表达水平,从而抑制心肌细胞凋亡改善心功能,是科素亚治疗心力衰竭的重要机制之一。
【关键词】心力衰竭心肌细胞凋亡 Bcl-2 科素亚
Abstract:Objective To study the effects of cozaar therapy on myocardial apoptosis and expressions of proapoptotic protein Bax and antiapoptotic protein
Bcl-2 in rats with chronic pressure overload heart failure (CHF), and its mechanisms. Methods 30 rats were randomly divided into three groups: CHF group, treatment group and sham operation control group (n=10 each). The rat model of CHF was induced by applying transverse abdominal aortic constriction. Treatment was started 6 weeks after the surgery, with cozaar 30 mg/kg . the model rats in treatment group, and with NS for the other 2 groups. By the end of 3 months, the hemodynamic parameters were determined, before the rats were killed to remove the heart. The hearts were dissected and weighed, the apoptosis was assessed by u