文档介绍：特发性血小板减少性紫癜与EB病毒感染的关系
【摘要】目的探讨特发性血小板减少性紫癜(ITP)与EB病毒(EBV)感染的相互关系。方法采用血清酶联免疫吸附试验(ELISA)及实时荧光定量聚合酶链反应(FQ-PCR)技术,对68例ITP和14例健康儿童血清EBV衣壳抗原IgM (EBV-CA-IgM)及外周血单核细胞内EBV-DNA进行定量检测,并结合临床进行分析。结果 68例ITP病儿EBV感染30例(44%),其中EBV-DNA(-)/EBV-IgM(+)24例,EBV-DNA(+)/EBV-IgM(+)6例,EBV-DNA含量每升(±)×108拷贝;6例EBV-DNA(+)病儿血清EBV-CA-IgM阳性5例,另1例EBV-CA-IgM由阴性转为阳性。正常对照组血清EBV-CA-IgM及EBV-DNA均阴性。EBV感染组、EBV-DNA(-)/EBV-IgM(+)组及EBV-DNA(+)/EBV-IgM(+)组与非EBV感染组ITP病儿外周血的白细胞数比较差异有统计学意义(F=,q=~,P<); EBV-DNA(-)/EBV-IgM(+)组与EBV-DNA(+)/EBV-IgM(+)组ITP病儿外周血的白细胞数差异无统计学意义(q=,P>)。非EBV感染ITP存在T淋巴细胞亚群的异常,即CD4+细胞降低,CD8+细胞升高。EBV感染ITP病儿尤其EBV-DNA(+)/EBV-IgM(+)组病儿T淋巴细胞总数亦降低,CD4+细胞降低明显。结论 EBV感染ITP存在T淋巴细胞免疫功能下降及紊乱,应同时进行抗病毒和免疫治疗。其血小板恢复时间长,但预后好。
【关键词】 Epstein-Barr病毒紫癜血小板减少性聚合酶链反应 T淋巴细胞
[ABSTRACT]ObjectiveTo study the correlation between Epstein-Barr Virus (EBV) infection and idiopathic trombocytopenia purpura (ITP). MethodsSerum EBV-CA-IgM and EBV-DNA in peripheral blood mononuclear cells in 68 cases with ITP and 14 healthy children were detected by enzyme linked immunosorbent assay(ELISA) and fluorescence quantitative-polymerase chain reaction (FQ-PCR) and analyzed. ResultsIn 68 ITP, 30 with EBV infection, in which, 24 with EBV-DNA(-)/EBV-IgM(+); six with EBV-DNA(+)/EBV-IgM(+); the content of EBV-DNA was (±)×108 Copy/L; five with both EBV-DNA(+) and EBV-IgM(+) in the beginning, only one with EB