文档介绍：组织工程骨复合骨形态发生蛋白修复兔颅骨缺损
作者:艾玉峰柳大烈熊猛郭树忠
【关键词】成骨细胞
关键词: 生物医学工程;聚羟基乙酸;成骨细胞;胶原;骨形态发生蛋白质类
摘要:目的探讨组织工程化骨修复兔颅骨缺损的实用价值及合理应用BMP的方法. 方法将自体成骨样细胞即刻种植在复合BMP的胶原包埋的聚羟基乙酸(PGA)的基质材料和不含BMP的基质材料上,然后将不同的复合体或单纯基质材料移植到兔颅骨的一侧全层骨缺损区,作为实验侧I、实验侧Ⅱ或实验侧Ⅲ.对侧设对照,,6,8及12wk处死,标本行大体组织学检查,X线摄片及灰量测定检查. 结果表明实验侧I成骨量明显大于实验侧Ⅱ或实验侧Ⅲ(P<),而对照侧仅为纤维组织修复. 结论组织工程骨可修复自体的兔颅骨缺损,应用外源性BMP可促进骨再生,为组织工程化骨在颅面外科及整形外科领域的临床应用奠定了基础.
Keywords:biomedical engineering;polyglycolic acid;os-teoblasts;collagen;bone ic proteins
Abstract:AIM To explore the practical value of repairing the defects of rabbit calvarial with tissue engineering tech-nique and the way of rational application of The osteoblast-like cells were instantly implanted onto the pounded polyglycolic acid(PGA)matrix coated with collagen and pure PGA matrix coated with rabbits were divided into three groups random- Group One,posite of osteoblast-like cells and PGA matrix with BMP was grafted into the defect on one side of the skull,leaving the same defect area on the other side as controls without any graft Group Two,cells+PGA were grafted in the same way as Group the third group,only PGA was grafted in the same way as Group rabbits were killed respectively on the2nd,6th,8th and the12th macroscopic examination,histol-ogy,x-ray and measurement of the ash wei