结核性胸腔积液中Th17细胞的水平及其意义.doc

文档介绍：
结核性胸腔积液中 Th17 细胞的水平及其意
义
罗丹1,2,孟婕1,2,王婷婷1,2**
5
10
(1. 南京大学医学院免疫学实验室;
2. 江苏省医学分子技术重点实验室,南京 210093)
摘要:目的:研究 Th17 细胞在结核性胸腔积液中的水平及意义。方法:分别提取结核病人
组的胸腔积液和外周血单个核细胞(PBMC),以及健康对照组的 PBMC。利用流式细胞术
检测 Th17 细胞占总 CD4+细胞的百分比。提取总 RNA,定量 PCR 检测 Th17 细胞相关因子
的 mRNA 表达水平。结果:结核性胸腔积液病人 Th17 细胞水平更高。结核性胸腔积液病人
Th17 细胞相关因子 mRNA 的水平也明显增多。结论:Th17 细胞在结核性胸腔积液的免疫
反应中发挥潜在作用,有望作为一个新的临床诊断方法。
关键词:Th17 细胞;结核性胸腔积液;细胞因子
中图分类号:
15
Increased frequencies of T helper type 17 cells in
tuberculous pleural effusion
1,2 1,2
1,2
(1. Immunology and Reproduction Biology Lab;
20
25
30
35
2. Medical School and State Key Laboratory of Pharmaceutical Biotechology,Nanjing
University,Nanjing 210093)
Abstract: Purpose: The current study was designed to investigate the possible involvement of Th17
cells in tuberculous pleural effusion. Methods: Pleural effusion and peripheral blood mononuclear cells
(PBMC) were extracted from TB group and healthy control group. The population of Th17 cells as a
percentage of total CD4+ cells was evaluated by flow cytometric analysis. The cell-free RNA was
extracted from effusions and the mRNA expression of Th17 cell-related factors was detected using
quantitative PCR. Results: Patients with tuberculous pleural effusions have a higher frequency of Th17
cells. Furthermore, the mRNA and protein expression levels of Th17-related factors (IL-17, IL-6) were
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