文档介绍：高效液相色谱仪的研制与技术开发
--新型二极管阵列检测器
洪群发张庆和李彤张维冰张玉奎
(大连依利特分析仪器有限公司,中国科学院大连化学物理研究所,大连,116011)
摘要:介绍一种新型的高效液相色谱二极管阵列检测器。该仪器采用光纤传导技术和全封闭光学系统,具有较高的光谱分辨率和检测灵敏度。采用虚拟设备驱动技术配合功能强大的数据处理系统可为用户提供色谱、光谱,三维谱图及色谱峰纯度等大量的信息。
关键词:高效液相色谱;二极管阵列检测器;虚拟设备驱动
Research and Developmemt on High Performance Liquid Chromatographic Instruments
--A Novel Diode Array Detector
Hong Qunfa, Zhang Qinghe, Li Tong, Zhang Weibing, Zhang Yukui
(Dalian Elite Analytical Instrument Ltd. Dalian Institute of Chemical Physics, Chinese Academy of Science, Dalian, 116011)
Abstract: A novel diode array detector (DAD) for high performance liquid chromatography (HPLC) was introduced. A full-closed optical system and quartz light fiber based transmission technique were used in the instrument, and high spectral resolution and detect sensitivity were obtained. Virtual device driving technique was used, bined with the powerful data system, the instrument can provide a lot of information including chromatogram, spectra ponents, three dimension information and peak purity results.
Key words: High performance liquid chromatography; Diode array detector; Virtual device driving
1. 前言
液相色谱分析中需根据被分析/分离的混合物组分分子结构的不同,使用相应的检测系统[1]。对于有光谱吸收的样品,只有在其有最大吸收的波长进行检测才可能得到最大的检测灵敏度。与传统紫外检测器相比,二极管阵列检测器(Diode Array Detector, DAD)可以同时给出光谱和色谱谱图,便于组分的定性和定量。八十年代初HP公司首次推出二极管阵列检测器[2],此后,各液相色谱仪器生产厂家相继推出多种同类产品,供与各种液相色谱仪配套选择,并不断向着提高灵敏度、改善检测器自身的选择性及减小谱带扩展的方向发