基于荧光共振能量转移的双酚A痕量检测法.doc

文档介绍：
基于荧光共振能量转移的双酚 A 痕量检测
法
聂雨,王周平**
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(江南大学食品学院,江苏无锡 214122)
摘要:以荧光纳米材料 KGdF4:Tb3+和纳米金为载体,实现对双酚 A 的痕量检测。将双酚 A
适配子及其互补的短链 DNA 分别与荧光纳米材料和纳米金进行连接,通过两条单链 DNA 的碱
基互补配对,两种材料结合在一起并发生荧光共振能量转移效应,纳米材料的荧光被纳米金
所淬灭。当体系中加入双酚 A 时,双酚 A 将与互补 DNA 链竞争结合双酚 A 适配子,从而导致
两种纳米材料结合量的不同,进而影响体系的荧光强度。通过测定双酚 A 的浓度与体系荧光
强度的线性关系,实现对双酚 A 的检测。在最优实验条件下,本法在双酚 A 浓度 ~100 ng/mL
范围内呈现良好的线性关系,回归方程为 Y=+75095(R²=),检出限为 ng/mL。
并通过加标回收实验,证明本法可用于实际样品的检测。
关键词:荧光能量共振转移;纳米金;双酚 A
中图分类号:
A FRET-based trace level detection method for Bisphenol A
Nie Yu, Wang Zhouping
(School of Food Science and Technology,Jiangnan University,JiangSu WuXi 214122)
Abstract: To set up a trace level detection method of bisphenol A based on the fluorescence
resonance energy transfer effect of KGdF4 and AuNPs. The bisphenol A aptamer and the aptamer
complementary strand were connected to the surface of KGdF4 and AuNPs. These two kinds
nanoparticles were connected to each other through plementary base pairing of two
ssDNA strands and this caused the effect of FRET that the fluorescence of KGdF4 was quenched
by AuNPs. When bisphenol A was added into the system, peted with the aptamer
complementary strand for binding to the bisphenol A aptamer which led to the different binding
rates of two nanoparticles. Different binding rates brought different fluorescence intensity of the
system.