参芪复方对GK大鼠肾脏NF-κBmRNA 和PPARγmRNA表达的影响.doc

文档介绍：
参芪复方对 GK 大鼠肾脏 NF-κBmRNA 和
PPARγmRNA 表达的影响#
郭保根1,孙凤平2,殷丽平1,谢春光1**
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(1. 成都中医药大学临床医学院;
2. 厦门市第一医院内分泌科)
摘要:目的:观察参芪复方对 GK 大鼠肾核转录因子κBmRNA(NF-κBmRNA)和过氧化
物酶体增殖物激活受体γmRNA(PPARγmRNA)表达的影响及其可能的作用机制。方
法:将随机血糖≥ 的 GK 大鼠 40 只随机分为雷米普利组、参芪复方高剂量组、
参芪复方低剂量组、模型组四组,设正常 SD 大鼠 10 只作为空白组。8 周后以 RT-PCR 法测
定大鼠肾 NF-κBmRNA和 PPARγmRNA的表达。结果:参芪复方高剂量组 GK 大鼠肾 NF-
κBmRNA 表达下降,PPARγmRNA 表达增强,和模型组比较有统计学意义(P<),且
二者呈负相关。结论:参芪复方高剂量能够抑制 NF-κB 的表达,增强 PPARγ活性,可能
是其抑制 DN 发生、发展的作用机制之一。
关键词:参芪复方;GK 大鼠;NF-κBmRNA;PPARγmRNA
中图分类号:请查阅《中国图书馆分类法》
Effects Of pound Recipe On Nuclear Factor
κBmRNA And PPARγmRNA In Kidney Of GK Rats
GUO Baogen1, SUN Fengping2, YIN Liping1, XIE Chunguang1
(1. College of Clinical Medicine,Chengdu University of TCM;
2. Department of endocrinology,the first hospital of Xiamen City)
Abstract: To observe the effects of pound Recipe on the express of NF-κBmRNA and
PPARγmRNA in Kidney of GK Rats and its possible mechanisms. Methods:The 40 GK rats, RBG≧
, were randomly divided into Ramipril group, the pound Recipe high dose
group, the pound Recipe low dose group, and model group. 10 normal SD rats were set as
the control group. After administered for 8 weeks, the expression of NF-κBmRNA and PPARγ
mRNA were determined from right kidney by RT-PCR way. Results:The expression of NF-κ
BmRNA in the Kidney of the pound Recipe high dose gr