文档介绍：
柠条锦鸡儿 CkLEA1 基因克隆及表达分析#
杨杞,尹佳佳,王颖,王瑞刚,李国婧**
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(内蒙古农业大学生命科学学院,呼和浩特 010018)
摘要:植物受到逆境胁迫后,大量逆境响应基因会被诱导表达,LEA 蛋白编码基因就是与
植物抗旱、抗冷等非生物胁迫密切相关的一类基因。从已构建的柠条锦鸡儿干旱胁迫抑制性
削减杂交文库中筛选到了一条 LEA 蛋白编码基因并进行了克隆。序列比对与系统进化分析
显示该基因属于 LEA3 基因家族成员,命名为 CkLEA1 (GeneBank 登录号是 KC309408)。克
隆得到该基因 gDNA 长 469bp,包含两个外显子和一个内含子;cDNA 长 357bp,包含 300bp
的开放阅读框,推导编码 99 个氨基酸的蛋白质。利用荧光定量 PCR 技术对 CkLEA1 基因在
各种逆境胁迫条件的表达情况进行初步研究表明,CkLEA1 受干旱、ABA、冷、热、盐和碱
等处理不同程度地诱导,推测其与柠条锦鸡儿响应逆境胁迫的机制有关。
关键词:LEA;基因克隆;表达分析;柠条锦鸡儿
中图分类号:Q94
Cloning and Expression Analysis of CkLEA1 Gene in
Caragana korshinskii Kom.
Yang Qi, Yin Jiajia, Wang Ying, Wang Ruigang, Li Guojing
(College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018)
Abstract: Under stress conditions, a lot of genes responsive to stress will be induced in plants.
Late-embryogenesis-abundant proteins (LEA protein) belong to an important protein family
related to drought, cold and other abiotic stress. An LEA encoding gene was isolated from the
suppression subtractive hybridization (SSH) library of Caragana korshinskii Kom. under drought
stress. Sequence and ic analysis indicated that it belongs to the LEA3 gene family, and
was named CkLEA1 (GenBank accession no. KC309408). The full length gDNA of CkLEA1 was
496bp, contained two exons and one intron. The full length cDNA was 357bp, and the protein
deduced from this p