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早熟菜心核基因雄性不育系选育及利用.doc

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早熟菜心核基因雄性不育系选育及利用.doc

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早熟菜心核基因雄性不育系选育及利用.doc

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早熟菜心核基因雄性不育系选育及利用#
姜明雪,冯辉**
(沈阳农业大学园艺学院,沈阳市 110161)
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摘要:本研究设计了一个“定向转育方案”转育复等位基因雄性不育系,以解决杂种生产难
题。以复等位基因遗传的小白菜核基因雄性不育系‘00S107’为不育源,采用回交转育的方
法向早熟菜心自交系‘06-1'中转育核不育基因,育成了不育株率和不育度均为 100%的菜
心核基因雄性不育系。利用‘GMS204' 配制杂交组合通过品种比较试验筛选出 3 个强优势
组合。
关键词:蔬菜学;菜心;核基因雄性不育系;转育
中图分类号:
Breeding and Utilization of an early-maturing ic male
sterile line in flowering Chinese cabbage
JIANG Mingxue, FENG Hui
(Department of Horticulture,Shenyang Agricultural University,Shenyang,110161)
Abstract: To produce hybrid seeds of Flowering Chinese cabbage, a "directional transfer program"
was designed to breed the multiple-allele male sterile line of Flowering Chinese cabbage. A
multiple-allele inheritance male-sterile line of Pakchoi (‘00S107’) was used as a source of male sterility,
and a recurrent backcrossing method was employed to transfer the male-sterile genes from ‘00S107’ to
a self-line of flowering Chinese cabbage (‘06-1’). This led to a new ic line of flowering Chinese
cabbage with 100% male-sterility made up entirely of males. This new line was applied to develop
binations, and four binations were selected..
Key words: Vegetable; Flowering Chinese Cabbage; multiple-allele male sterile line; Breeding
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0 引言
菜心(Brassica campestris L. ssp. chinensis var. utilis Tsen et Lee)是十字花科芸苔属芸薹
种蔬菜作物,以花薹和嫩叶为产品器官,是我国华南地区主栽蔬菜之一。菜心异花授粉,具
有显著的杂种优势。由于其花器官小,单花结籽少,而单位面积的播种量