文档介绍：基因组学与应用生物学,2013年,第32卷,第5期,第612-615页Genomics and Applied Biology, 2013, , , 612-615研究报告Research Report一株高浓度烟碱降解菌的筛选、分离和初步鉴定龙章德1韦方立2李俊芳3田兆福1张敏3黎新钦1申佩弘3*1广西中烟工业有限责任公司,南宁, 530001; 2广西民族师范学院化学与生物工程系,崇左, 532200; 3广西大学生命科学与技术学院,南宁,530004*通讯作者, shenpeihong1977@摘要采用以烟碱为唯一碳源氮源的选择培养基,从烟草和植烟土壤中分离筛选出15株高浓度烟碱降解的菌株,其中菌株D9烟碱降解能力最强,其烟碱降解率为82%,耐受烟碱的最高浓度为8 g/L。经常规形态特征、16S rDNA同源序列以及系统进化树分析,初步鉴定该菌株为类似氧化微杆菌,。本研究为微生物降解烟碱的研究及应用提供了菌种资源。关键词烟碱,生物降解,烟叶,微杆菌Screening, Isolation and Identification of a High Concentration NicotineDegradation BacteriumStrainLong Zhangde1Wei Fangli2Li Junfang3Tian Zhaofu1Zhang Min3Li Xinqin1Shen Peihong3*1 China o Guangxi Industrial Co., Ltd., Nanning, 530001; 2 Chemical and Biological Engineering Guangxi Normal University for Nationali-ties, Chongzuo, 532200; 3 College of Life Science and Technology, Guangxi University, Nanning, 530005* Corresponding author, ******@DOI: .000612Abstract15 nicotine-degradingbacterial strains were separated and screened o and soil with selectivityculture medium, strain D9 was the best one with the highest nicotine degradation ratio reached at 82%, and couldgrow in a high nicotine concentration reached at 8 g/L using nicotine as the sole carbon and nitrogen source. Thestrain D9 was identified asMicrobacteriumsp. GYC29 according to the normal morphology, physiological and bio-chemical c