文档介绍：基因组学与应用生物学,2013年,第32卷,第2期,第207-211页Genomics and Applied Biology, 2013, , , 207-211研究报告Research Report吉富罗非鱼群体遗传多样性的AFLP分析燕涛郭昱嵩王中铎刘丽刘楚吾*广东海洋大学水产学院,南海水产经济动物增养殖广东普通高校重点实验室,湛江, 524025*通讯作者, ******@,为其育种提供理论依据,本实验利用5对引物组合(E-AGG/M-CTT, E-AGG/M-CTG, E-AAC/M-CAG, E-ACA/M-CTG, E-ACA/M-CAG)对广东省化州光辉养殖场有限公司新选育的吉富罗非鱼第16代群体(F16)进行遗传多样性的AFLP分析。27个个体共检出187个扩增位点,其中多态位点163个,,%;平均Nei' 6;平均Shannon' 9。结果说明实验群体存在较丰富的遗传多样性且保持有较高的遗传杂合度,具有进一步育种和开发的价值。关键词吉富罗非鱼,扩增片段长度多态性(AFLP),遗传多样性,ic Diversity of GIFT Tilapia Populations by AFLP AnalysisYan Tao Guo Yusong Wang Zhongduo Liu Li Liu Chuwu*Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College,Guangdong Ocean University, Zhanjiang, 524025* Corresponding author, ******@: .000207AbstractThe experiment was conducted to assess the ic diversity of ically improved farmed tilapia(GIFT) in order to evaluate and preserve this strain as a germplasmresource. The experiment study focused on ic diversity of the newly bred F16population of GIFT tilapia obtained fromGuanghui pany,Huazhou(Guangdong, China) was analyzed usingAFLP. 187 bands were detectedwith5 pairs of AFLPprimers basedon 27 individuals, and among them163 (%) were polymorphic with an average of bands for per AFLPprimer