鸡J亚型禽白血病病毒检测及相关分析.doc

文档介绍：
鸡 J 亚型禽白血病病毒检测及相关分析#
兰茜,尹月,李天杰,朱庆,刘益平,赵小玲,王彦**
(四川农业大学动物遗传育种研究所,四川雅安,625014)
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摘要:目的】对四川省某鸡场鸡群进行 J 亚群禽白血病病毒(ALV-J)感染筛查,并进行 ALV-J
病毒感染与蛋鸡产蛋性能的相关性研究。【方法】随机抽检 348 只鸡的血样,提取总 DNA,
利用 PCR 扩增目的基因 ALV-J,PCR 产物用 1%的琼脂糖电泳检测。【结果】该鸡群的感
染率为 %;同时,ALV-J 阳性个体在大部分产蛋性能方面显著低于 ALV-J 阴性个体:
开产体重和后期体重分别显著降低 %和 %(P<)。同时,ALV-J 阳性个体较阴
性个体在 1 期累计产蛋量显著下降(P<);且 ALV-J 感染鸡群产蛋高峰持续时间短。
【结论】J 亚群禽白血病毒 ALV-J 对鸡的产蛋性能造成了严重的负面影响,感染鸡只的产蛋
性能显著降低。
关键词:蛋鸡;J 亚型禽白血病;产蛋性能
中图分类号:
Avian Leucosis Virus Subtype J detection and correlation
analysis
LAN Xi, Yin Yue, LI Tianjie, ZHU Qing, LIU Yiping, ZHAO Xiaoling, WANG Yan
(Institute of Animal ics and Breeding, Sichuan Agricultural
University,Ya'an,625014,Sichuan)
Abstract: 【Objective】The objective of this study was to screen ALV-J from a chicken farm in
Sichuan province, and to investigate the relationship of the infection of avian leucosis virus
subtype J and chicken laying performance.【Method】Total DNA was isolated from the blood of
348 chicken, which were randomly chosen from the farm. Polymerase chain reaction technique
was used to amplification the target gene ALV-J,and PCR products were confirmed by %
agarose gel electrophoresis.【Results】The results showed that the ALV-J positive rate were
%. Furthermore, ALV-J could reduce most laying performance of chicken. ALV-J
significantly reduced