文档介绍：基因组学与应用生物学,2013年,第32卷,第5期,第652-659页Genomics and Applied Biology, 2013, , , 652-659数据分析Data Analysis云南红皮梨bHLH转录因子的生物信息学分析孟富宣周军*辛培尧唐军荣董娇付海辉周林涛徐世宏西南山地森林资源保育与利用省部共建教育部重点实验室,西南林业大学,昆明, 650224*通讯作者, zhoujunbo@摘要具有碱性- 螺旋- 转角- 螺旋结构的一大类基因被称为bHLH家族基因,其成员主要有c-MYC、MADL、MAX、MIXL和MNT基因;bHLH转录因子在细胞的增殖、分化和凋亡方面起着重要作用;对植物的生长发育、营养吸收、生物合成和信号转导等方面同样有着重要作用。在果肉、叶片和外果皮中,CsMYC2的表达与CHS、UFGT及ANS表达相关联,因此CsMYC2参与花色苷的生物合成调控,但是该基因在云南红皮梨花色苷合成调控中起着什么样的作用尚不清楚。利用生物信息学方法对云南红皮梨(RedPyrus pyrifolia)bHLH基因进行分析,对不同植物间bHLH基因氨基酸序列的同源性比对,并且对该基因编码的蛋白质的理化性质、结构和功能等进行预测和分析。结果表明:该蛋白属于亲水性蛋白,其二级结构中的主要结构元件是琢- 螺旋和无规则卷曲,并散布于整个蛋白。这一结果为研究红皮梨bHLH基因的结构、功能以及红皮梨的着色机制奠定了一定的基础。关键词云南红皮梨, bHLH转录因子,生物信息学Bioinformatics Analysis of bHLH Transcription Factor in Yunnan Red SkinPear (Pyrus pyrifoliaNaki)Meng Fuxuan Zhou Jun*Xin Peiyao Tang Junrong Dong Jiao Fu Haihui Zhou Lintao Xu ShihongKey Laboratory for Forest Resource Conservation and Use in the Southwest Mountains of China, Ministry of Education, Southwest Forestry Univer-sity, Kunming, 650224* Corresponding author, ******@DOI: .000652AbstractThe bHLHa basic-helix-turn-helix structure and its bHLHfamily members incloudc-MYC,MADLandMAX,MIXLandMNT. bHLH transcription factors play an important role in cell proliferation, differentiation andapoptosis. It also has a vital role on plant growth and development, nutrient absorption and biosynthesis, signaltransduction, etc. In the flesh, leaves and exocarp,CsMYC2expression was associated withCHS,UFGTandANSexpression. Therefore,CsMYC2involved in anthocyanin biosynthesis and regulation. It is not clear what the geneplays a role of anthocyanin synthesis regulation in Yunnan red-skin pear. This research investigated thebHLHgeneof Yunnan red-skin pears by using bioinformatic method and software. The homology of amino acid sequences ofthe bHLHamong different plants pared and the physical and chemical properties, structure and function ofthe gene encoding protein were predicted and analyzed. The result showed that