文档介绍：Tissue Culture Technique
(In Vitro Culture)
I. Introduction
Ⅱ. Types of cell culture
Ⅲ. Equipment
Ⅳ. Cleaning and Disinfection
Content
I. Introduction
history
(1) In vitro culture
whole culture
organ culture
parts culture tissue culture
cell culture
Organ culture
The maintenance or growth an primordia or the whole or parts of an in vitro,in a way that may allow differentiation and preservation of the architecture and/or function.
Tissue culture
The maintenance or growth of tissue in vitro,in a way that may allow differentiation and preservation of the architecture and/or function.
Cell culture
This term is used to denote the growing of cell in vitro,including the culture of single cell. In cell culture,the cells are no anized into tissues.
(2) In vivo culture
transplantation
stem cell
history
1885 Roux
1907 Harrison
1910 Carrel
1950s Aseptic technique
Antibiotics
Medium
Culture methods-Microcarrier
culture,and so on.
3. Application
(1) Biologic product (biologics)
(2) Virology
(3) Stem cell culture
(4) ics
(5) Cytotoxic test
(6) Immunology