文档介绍：工艺技术
紫阳富硒茶中硒蛋白的提取
高二东,董文宾
陕西科技大学生命科学与工程学院(西安710021)
摘要为了优化碱法提取硒蛋白的工艺条件,在单因素试验的基础上,通过Box-Benhnken中心组合试
验设计及响应面分析方法,考察了碱液浓度、提取温度以及料液比三因素对蛋白质提取率的影响,得出
了硒蛋白提取效果的回归模型。结果表明, mol/L, ℃,料液
比1∶(g:mL),每次提取3 h,提取2次,%, μg/g,
%。
关键词富硒茶;硒蛋白;提取工艺;响应面分析
Extraction of Selenium-protein from Ziyang Selenium-enriched Tea
Gao Er-dong , Dong Wen-bin
College of Life Science and Engineering, Shaanxi University of Science and Technology (Xi’an 710021)
Abstract In order to optimize extraction condition of Selenium-protein, according to the analysis method of center
combination test and response surface methodology, the effects of NaOH concentration, extraction temperature
and ratio of solid/liquid on Selenium-protein extraction were studied on the basis of the results of single factor
tests, the regression model of the extraction effect of Selenium-protein was established. The optimum technology
conditions were confi rmed as follows: concentration of NaOH slurry mol/L, extraction temperature ℃,
ratio of solid/liquid 1∶ (g:mL), extracting 3 h each time for 2 times. Under this condition, the yield of Selenium-
protein was % and the content of selenium was μg/g, accounting for % total selenium in tea.
Keywords selenium-enriched tea; selenium-protein; extracting technol