文档介绍：中国农业科学 2005,38(11):2308-2315
Scientia Agricultura Sinica
柚类种质资源 AFLP 与 SSR 遗传多样性分析
刘勇 1,2,孙中海 1,刘德春 2,吴波 2,陶建军 1
(1 华中农业大学柑桔研究所,武汉 430070;2 江西农业大学农学院,南昌 330045)

摘要:结合 SSR 引物和 AFLP 分子标记对 110 份柚类基因型、12 份野生近缘种进行遗传多样性研究。结果表
明,SSR 标记的 335 个位点中 %为多态性位点,每个位点可检测到个等位基因,基因多样度(GD)变幅
为 ~,获得了 46 个特异性 SSR 标记;AFLP 标记的 343 个位点中 72%为多态性位点,3 对引物平均期
望杂合度变幅为 ~,平均每对引物组合能产生 82 个多态位点,获得了 44 个 AFLP 特异性标记。UPGMA
聚类结果显示,122 份基因型在相似系数时,分成 8 个组群,其中柚类主要由沙田柚品种群、文旦品种群与
庞大的杂种柚品种群组成。分类结果将有利于更好地利用这些丰富的育种资源。
关键词:柚类;种质资源;遗传多样性;SSR;AFLP

Assessment of the ic Diversity of Pummelo Germplasms
Using AFLP and SSR Markers
LIU Yong1, 2, SUN Zhong-hai1, LIU De-chun2, WU Bo2, TAO Jian-jun1
(1Citrtus Research Institute, Huazhong Agricultural University, Wuhan 430070; 2College of Agronomy,
Jiangxi Agricultural University, Nanchang 330045 )

Abstract: ic diversity of 110 pummelo germplasm and 12 its relatives were analyzed by SSR and AFLP. Approximately
% of the 335 SSR were polymorphic, and alleles per SSR loci were identified. Gene diversity values changed from
to , and 46 SSR polymorphic bands were scored. 72% of the 343 AFLP were polymorphic, and 82 polymorphic loci per AFLP
were identified. Heterozygosity changed from to , and 44 AFLP polymorphic bands were scored. The UPGMA result
showe