单癸月桂酸甘油酯的抑菌作用及机理研究.docx

文档介绍：70 2013,, 食品科学 ※基础研究
单癸月桂酸甘油酯的抑菌作用及机理研究
曾哲灵1,一,赵存洋1’3,罗春燕。3,周星1’3
(,江西南昌 330047:,江西南昌 330031;
,江西南昌 330031)
摘要:对不同质量浓度的单癸月桂酸甘油酯的抑菌活性及其抑菌机理进行研究。采用滤纸片法对单癸月桂酸甘油酯进行细菌敏感性测试;二倍试管稀释法测定最低抑菌质量浓度(MIC):光电比浊法测定抑菌效果;二乙酸荧光素(FDA)染色实验研究其对细菌膜通透性的影响;分光光度法测定细菌胞内蛋白含量、细菌细胞壁脂多糖(LPS)含量、细菌菌悬液离心后上清液中核酸物质含量;原子吸收光谱法测定细菌菌悬液离心后上清液中胞内内容物K+、Ca“、
M92+含量;扫描电镜观测细菌表面形态变化。结果表明:单癸月桂酸甘油酯对革兰氏阳性菌及阴性菌均有较好的抑制效果,对金黄色葡萄球菌(G+)、枯草芽孢杆菌(G+)的抑制作用主要是破坏其细胞膜及细胞壁,对大肠杆菌(G一)的抑制作用主要是引起细胞壁外膜LPS夕b泄。
关键词:单癸月桂酸甘油酯;抑菌作用;抑菌机理
Antibacterial Function and Mechanism of Monolaurin and Monocaprin
ZENG Zhe—lin91'2,ZHAO Cun—yan91’3,LUO ,3,ZHOU Xin91'3
( Key Laboratory ofFood Science and Technology,Nanchang University,Nanchang 330047,China; of Environmental and Chemical Engineering,Nanchang University,Nanchang 330031,China; of Life Science and Food Engineering,Nanchang University,Nanchang 330031,China)
Abstract:The objective of this study is to explore the antimicrobial activity and mechanism of monoglycerides containing
monolaurin and disc method was used to evaluate bacterial susceptibility of monolaurin and monocaprin. Two—fold dilution method was used to test minimum inhibitory concentration(MIC),and photoelectricity turbidimetry method was used to determine antibacterial efficacy。Orescein diacetate(FDA)was used as fluo