文档介绍：口腔生物医学2011 年6 月(第2 卷第2 期) Oral Biomedicine,Vol 2,No 2,Jun 2011 ·75·
[文章编号] 16748603(2011)02007503
白色念珠菌人工诱导耐药模型的建立及鉴定方法的研究
虞丽华,马鸣,陈晓君,徐双波,魏昕
(南京医科大学口腔医学研究所·附属口腔医院牙体牙髓病科,江苏南京210029)
[摘要] 目的:利用氟康唑人工诱导构建白色念珠菌耐药模型。方法:采用梯度浓度递增法体外氟康唑诱导白色念珠菌耐药
株,采用KONT 真菌显色MIC 药敏系统以及药敏纸片扩散法两种方法对构建形成的耐药菌株进行检测。结果:氟康唑人工诱
导白色念珠菌耐药株构建形成,经微量稀释法鉴定最低抑菌浓度(minimal inhibitory concentration,MIC)≥ 64 μg / ml。KONT 真
菌显色MIC 药敏系统及药敏纸片扩散法判定结果符合率达95% ,P > 0. 05,无统计学差异。结论:采用梯度浓度递增法体外诱
导白色念珠菌耐药模型可行,且KONT 真菌显色MIC 药敏系统与药敏纸片扩散法对耐药株的鉴定结果一致性较好。
[关键词] 白色念珠菌;人工诱导耐药;KONT 真菌显色MIC 药敏系统;药敏纸片扩散法
[中图分类号] R780. 2 [文献标识码] A [doi] 10. 3969 / j. issn. 16748603. 2011. 02. 005
Establishment of resistant model in Candida albicans with induction and identified of resistanceinduced isolates
YU Lihua,MA Ming,CHEN Xiaojun,WEI Xin. (Insititute of Stomatology,Department of Operative Dentistry and Endodontics,School of
Stomatology,Nanjing Medical University,Nanjing 210029,China)
Corresponding author:WEI Xin,Email:weixinart@ 163. com,Tel:00862585031822
[Abstract] Objective:Induce resistant isolate in Candida albicans by fluconazole(FLU). Methods:Drug resistance was induced in
vitro by a multistep method in Candida albicans strain SC5314 with FLU,and the susceptibilities of resistant strains were determined
by KONT MIC determination system and disc diffusion test. Results:Isolates that developed FLU≥ 64 μg / ml aft