文档介绍：昆布水提物对人子宫内膜癌细胞系BMDC1(ADR+/+)多药耐药性的影响
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作者:李巧敏,连易水,张静,单保恩,赵瑞撵,沈莉
【摘要】目的:研究昆布提取物(TLPE)在体外对耐药细胞BMDC1(ADR+/+)的逆转作用,探讨其逆转肿瘤多药耐药的机制. 方法:应用流式细胞术测定不同剂量TLPE对BMDC1用药前后细胞内蛋白激酶Cα(PKCα)和谷胱甘肽转移酶π(GSTπ)的表达变化,测定细胞周期和凋亡率;应用姬姆萨染色技术观察细胞形态学改变及细胞凋亡情况;应用比色法检测各组细胞ATP酶(ATPase)变化. 结果:TLPE在70~300 mg/L浓度范围内能明显降低BMDC1细胞内PKCα和GSTπ的表达(),并将细胞阻滞于S期,但不诱导细胞凋亡;TLPE对细胞内ATPase无影响(). 结论:TLPE对子宫内膜癌细胞系BMDC1(ADR+/+)具有耐药逆转作用,其逆转作用与其诱导PKCα和GSTπ的表达降低有关.
【关键词】昆布水提物;子宫内膜肿瘤;多药耐药
【Abstract】 AIM: To study the multidrug resistance (MDR)
reversing effect of Chinese drug Thallus Laminariae PE (TLPE) on human endometrial cancer cell line BMDC1 (ADR+/+) in vitro and its underlying mechanism. METHODS: The expressions of PKCα and GSTπ at the level of protein in BMDC1 cell treated with different doses of TLPE were analyzed by flow cytometry. The cell cycle and apoptosis rate of BMDC1 (ADR+/+) cells treated by TLPE were detected. Their morphological changes were observed by Gimsa staining methods. Colorimetry was used to detect the changes of ATPase on BMDC1 (ADR+/+) cells treated by TLPE. RESULTS: After interfered by TLPE (70-300 mg/L), the expressions of PKCα and GSTπ in cells were decreased significantly (). TLPE could block cell cycle at S period, but it did not obviously induce cell apoptosis. There was no significant change in the