小麦BR合成基因细胞色素P450基因（TaDWARF4）的克隆及序列分析.doc

文档介绍：
小麦 BR 合成基因细胞色素 P450 基因
(TaDWARF4)的克隆及序列分析#
张文萍1,张磊1,周宇飞1,韩雷2**
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(1. 沈阳农业大学农学院,沈阳,110866;
2. 沈阳农业大学实验场种子服务公司,沈阳,110866)
摘要:油菜素内酯(BRs)作为植物的第六大激素调控着植物的诸多生长发育过程。研究小
麦油菜素内酯相关基因对小麦品种的改良具有重要意义。本研究克隆了小麦 BR 合成后期
基因 TaDWARF4,通过测序得到该基因在普通小麦中的 A、B 和 D 三个基因组序列。分析
三个基因组的结构,发现三个基因组在编码区存在差异,编码的蛋白存在差异,可能具有
不同的功能。根据 D 基因组序列设计特异引物,利用中国春缺体-四体将该基因定位在第二
同源群上。通过构建系统进化树分析发现,该基因与禾本科处于同一分支,与拟南芥较
远,说明该基因的结构比较保守。可能与亲缘关系较近的禾本科作物水稻具有相似的功能。
关键词:小麦;油菜素内酯;TaDWARF4 基因
中图分类号:
Cloning and sequnce analysis of BR synthesis cytochrome
P450 gene (TaDWF4) in bread wheat
Zhang Wenping1, Zhang Lei1, Zhou Yufei1, Han Lei2
(1. College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China;
2. Seed service department of experimental farm,Shenyang Agricultural University,Shenyang
110866, China)
Abstract: Brassinosteroids (BR) is the sixth plant hormone. It is significant to study the
brassinosteroids related genes to improve the varieties and increase the yield of bread wheat. In
the present study, the BR synthesis gene TaDWARF4 was cloned and A, B, D genomes were
obtained by sequence. There were differences in the exons by analyzing the structure of the three
genomes, and they might have different functions due to the differences in protein sequences. A
pair of D genome specific primers were designed, and the genes were localized in the second