小麦光周期相关基因（TaPRR73）的克隆及生物信息学分析.doc

文档介绍：
小麦光周期相关基因(TaPRR73)的克隆及
生物信息学分析#
张文萍1,张磊1,周宇飞1,韩雷2**
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(1. 沈阳农业大学农学院,沈阳,110866;
2. 沈阳农业大学实验场种子服务公司,沈阳,110866)
摘要:光周期是植物适应环境的一种现象,能够通过日照长度调控植物的抽穗期。小麦是严
格的长日照作物,且种植面积广泛,研究小麦中的光周期基因对提高小麦品种对不同光照条
件的适应性,提高小麦产量等具有重要意义。本研究同源克隆了小麦的光周期相关基因
TaPRR73,通过测序得到该基因在中国春上的 gDNA 及 cDNA 全长序列。根据 A、B、D 三
个基因组序列存在的插入缺失设计染色体定位引物,利用小麦缺体-四体系列将该基因定位
在小麦第四同源群上。对该基因进行结构预测,发现该基因具有 PRRs 家族的两个保守域: 受
体结构域(Pseudo-receiver domain)T,说明该基因结构比较保守,可能在
调节小麦光周期中发挥功能。
关键词:小麦;光周期;TaPRR73 基因
中图分类号:
Cloning and bioinformatics analysis of wheat photoperiod
related gene (TaPRR73) in bread wheat
Zhang Wenping1, Zhang Lei1, Zhou Yufei1, Han Lei2
(1. College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China;
2. Seed service department of experimental farm,Shenyang Agricultural University,Shenyang
110866, China)
Abstract: Photoperiod is an important physiological phenomenon which is the result of the plant
adapts to the different environments, and it controls flowering time by receiving day length. In the
present study, a novel gene TaPRR73 was cloned from hexaploid wheat by using homologous
cloning method. The chromosomes location primers were designed according to the indel
polymorphisms in the three genomes, and the gene was localized on the fourth homologous group.
Bioinformatics analysis revealed TaPRR73 has two conserved domain by prediction the ge