文档介绍：
甘氨酸和菜籽油双抗法诱变选育多杀菌素
高产菌株#
王美玲,赵方龙,尹静,卢文玉**
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(天津大学化工学院生物工程系,系统生物工程教育部重点实验室,天津化学化工协同创新
中心,天津 300072)
摘要:结合多杀菌素生物合成途径和代谢调控理论,提出了基于甘氨酸和菜籽油双抗性的多
杀菌素高产菌推理选育方法。通过紫外诱变、亚硝酸钠诱变,结合甘氨酸和菜籽油双重抗性
筛选,获得突变株刺糖多孢菌 4/26,多杀菌素产量达到 mg/L,为出发菌株 49460
的 4 倍。
关键词:微生物学;多杀菌素;抗性选育;诱变;刺糖多孢菌
中图分类号:Q93
Glycine and cole seed oil resistance breeding of high
spinosad producing strains by mutation
WANG Meiling, ZHAO Fanglong, YIN Jing, LU Wenyu
(Collaborative Innovation Center of Chemical Science and Engineering, Key Laboratory of
system bioengineering, Ministry of Education, School of Chemical Engineering and Technology,
Tianjin University, Tianjin 300072)
Abstract: In this paper,an efficient method for breeding the high spinosad producing strains by
glycine and cole seed oil resistance mutation was conducted,according to the biosynthesis path of
spinosad. A high yield strain haropolyspora spinosa 4/26 was obtained by rational breeding of
the original wild strain 49460 with the UV and NaNO2 mutation. Spinosad yield was
, which was 4-fold higher than that of the wild 49460.
Key words: Microbiology ; Spinosad; resistance breeding; mutation; haropolyspora
spinosa
0 引言
多杀菌素(spinosad)是由刺糖多孢菌产生的次级代谢产物,具有杀虫广谱性、环境的
安全性以及良好的可降解性等优点,应用前景广泛。目前,制约多杀菌素大范围应用的瓶颈
在于生产成本高,发酵效能低。菌株生产能力提高是目前国内多杀菌素研究的重点。
常规理化诱变菌株虽然具有非定向性、工作量大等特点,但因其操作简便仍被广泛应用。
代鹏等[1]过紫外线、N+