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Gene Cloning and Manipulation,Second Edition
Now fully updated to reflect recent advances, this introduction provides a
broad, but concise, coverage of binant DNA techniques. Written for
advanced undergraduates, graduates and scientists who want to use this
technology, emphasis is placed on the concepts underlying particular
types of cloning vector to aid understanding and to enable readers to
devise suitable strategies for novel experimental situations. An introduc-
tion to the basic biochemical principles is presented first. Then PCR and
cloning using E. coli hosts and plasmid, phage and hybrid vectors are
described, followed by the generation and screening of libraries and how
to modify, inactivate or express cloned sequences. Finally, ic
manipulation in a range of anisms is discussed, including
other bacteria, fungi, algae and plants, insects and mammals. A series of
‘real-life’ biological problems is also presented to enable readers to assess
their understanding of the material and to prepare for exams.
Christopher Howe is Professor of Plant and Microbial Biochemistry
at the University of Cambridge. His research interests include the
biochemistry and molecular biology of anisms, and
the evolutionary processes that gave rise to them. He has taught
molecular biology for 20 years.
Gene Cloning and Manipulation
Second Edition
Christopher Howe
University of Cambridge
CAMBRIDGE UNIVERSITY PRESS
Cambridge, New York, Melbourne, Madrid, Cape Town, Singapore, São Paulo
Cambridge University Press
The Edinburgh Building, Cambridge CB2 8RU, UK
Published in the United States of America by Cambridge University Press, New York
Information on this title: 0521817936
© C. J. Howe 2007
This publication is in copyright. Subject to statutory exception and to the provision of
relevant collective licensing agreements, no reproduction of any part may take place
without the written permission of Cambridge Universit