文档介绍：Gene Cloning and Manipulation
Second Edition
Christopher Howe
University of Cambridge
CAMBRIDGE UNIVERSITY PRESS
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Cambridge University Press
The Edinburgh Building, Cambridge CB2 8RU, UK
Published in the United States of America by Cambridge University Press, New York
Information on this title: 0521817936
© C. J. Howe 2007
This publication is in copyright. Subject to statutory exception and to the provision of
relevant collective licensing agreements, no reproduction of any part may take place
without the written permission of Cambridge University Press.
First published in print format 2007
ISBN-13 978-0-511-29499-0 eBook (EBL)
ISBN-10 0-511-29499-9 eBook (EBL)
ISBN-13 978-0-521-81793-6 hardback
ISBN-10 0-521-81793-5 hardback
ISBN-13 978-0-521-52105-5 paperback
ISBN-10 0-521-52105-X paperback
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Contents
Preface to first edition page vii
Preface to second edition ix
Chapter 1 The tools for the job 1
Chapter 2 Polymerase chain reaction 30
Chapter 3 Simple cloning 52
Chapter 4 Other vector systems for E. coli 74
Chapter 5 Making libraries 98
Chapter 6 Screening libraries 116
Chapter 7 Modification and mutagenesis 143
Chapter 8 Use of cloned DNA 162
Chapter 9 Using anisms 182
Chapter 10 Examples 243
References 250
Index 257
Preface to first edition
This book grew out of sets of lectures given to undergraduates taking
courses in Biochemistry and Molecular Biology, and Medical Sciences.
I hope it will be useful to people studying a range of biological
subjects. I have tried to concentrate throughout on the general
principles underlying the subject rather than to give overwhelmingly
detailed accoun