文档介绍：基因组学与应用生物学,2013年,第32卷,第3期,第393-398页Genomics and Applied Biology, 2013, , , 393-398评述与展望Review and Progress化学交联法在DNA损伤和修复机制研究中的应用唐志姣1,2刘芳1,2王呈坤2龚福春1*1长沙理工大学化学与生物工程学院,长沙, 410114; 2中国科学院上海有机化学研究所生命有机化学国家重点实验室,上海, 200032*通讯作者, gfc139@摘要DNA分子在生物体内受到物理、化学等因素的影响,如X- 射线和药物,会发生不同程度与类型的碱基损伤,诱发基因突变或细胞损害,引发包括癌症在内的各种疾病。因此研究DNA损伤与修复机制至关重要。但在DNA损伤修复机制研究过程中,目前最大的困难在于如何有效架构碱基损伤的DNA与用于修复DNA损伤修复酶的瞬态复合物,以探讨这些酶如何特异性识别损伤位点。本文综述利用化学交联法通过二硫键的方式实现酶与碱基损伤的DNA瞬态复合物的形成的相关研究进展,以期为其它DNA损伤或DNA碱基化学修饰机制研究提供参考。关键词化学交联法, DNA损伤, DNA修复,二硫键The Application of Chemical Crosslinking in the Mechanism Study of DNADamage and RepairTang Zhijiao1,2Liu Fang1,2Wang Chengkun2Gong Fuchun1*1 College of Chemistry and Biochemical Engineering, Changsha University of Science and Technology, Changsha, 410114; 2 State Key Lab anic and Natural Products Chemistry, Shanghai Institute anic Chemistry, Chinese Academy of Sciences, Shanghai, 200032* Corresponding author, ******@DOI: .000393AbstractCellular DNA is constantly subjected to modifications by intracellular and extracellular chemicals,which can result in all kinds of damage, and then cause various diseases including cancer. To understand pro-tein/DNA interactions and the damage repair mechanism, a structure of the protein/plex is , transient protein/plexes are almost impossible to obtain for structural studies using traditionalmethods fo