文档介绍：高效液相色谱——二极管阵列检测法测定芫花药材指纹图谱
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作者:田元子李娆娆雷沛霖黄兰岚原思通刘营
【摘要】目的建立芫花药材的高效液相指纹图谱分析方法。方法采用DiamondC18色谱柱( mm × 250 mm, 5 μm),甲醇水梯度洗脱,体积流量为1 ml/min,柱温35 ℃,检测波长332 nm,记录时间50 min。以芫花素为参照物,对17批样品进行测定并计算相似度。结果初步建立芫花药材的指纹图谱,标定7个共有峰,各产地药材之间的相似性良好。结论该方法稳定可靠,简单可行,能够提高芫花药材的质量控制标准。
【关键词】芫花药材反相高效液相指纹图谱相似度
Abstract:ObjectiveTo establish the analytical method for the fingerprint of Flos Genkwa by HPLCDAD. Methods HPLC condition: column Diamond C18 ( mm × 250 mm, 5 μm), the mobile phase was mixtures of methanol and water in gradient elution mode, the flow was 1 ml/min, the column temperature was 35 ℃, the detection wavelength was set at 332 nm, and record time was 50 min. Genkwanin was used as the re
pound and 17 batches of Flos Genkwa were determined. Results7 copossessing peaks were selected as mon peaks, and the similarity of the fingerprint was close in different growing region. ConclusionThis method is simple, accurate and can provide scientific reference for further quality control of Daphne genkwa Sieb. et .
Key words:Flos Genkwa; HPLC-DAD; Fingerprint; Similarity
芫花为瑞香科植物芫花Daphne genkwa Sieb. et .的干燥花蕾,为传统的泻下逐水药,有毒,具有泻水逐饮、解毒杀虫的功效,内服用于治疗水肿胀满,胸腹积水,痰饮积聚,气逆咳喘,二便不利,外用治疗疥癣秃疮,冻疮[1]。现代研究表明芫花还具有治疗慢性支气管炎、肝炎、妊娠中期引产、抗白血病等作用[2]。
芫花中的黄酮类成分(芫花素等)为其镇咳祛痰、杀虫的有效成分;二萜原酸酯类成分(芫花酯甲等)是利水、杀虫的有效成分,也是毒性成分[2]。两种成分含量测定方法及含量限度要求已另文发表[3,4]。作为多组分的中药材,仅有某个有效成