文档介绍：第三军医大学
硕士学位论文
晶体蛋白对离体培养的大鼠视网膜神经节细胞的作用研究
姓名:王艳华
申请学位级别:硕士
专业:眼科学
指导教师:王一
20040501
第三军医大学硕士学位论文
英文缩写一览表

英文缩写英文全称中文名称
APS Ammonirm Persulfate 过硫酸铵
BDNF Brain-derived neurotrophic factor 脑源性神经营养因子
CNTF Ciliary neurotrophic factor 睫状神经营养因子
DAB Diaminobenzidine 二氨基联苯胺
DMEM o’s modified Eagle’s medium DMEM 培养液
DMSO Dimethyl sulfoxide 二甲基亚砜
DTT Dithiothreitol 二硫苏糖醇
FITC Fluorescein isothiacyanate 异硫氰酸荧光素
GM-CSF Granulocyte-macrophage colony stimulating 粒-巨噬细胞集落刺激因子
factor
HEPES Hydroxyethyl piperazine ethanesulfonic N-2 羟乙基哌嗪-N'-2-乙磺酸
acid
IGFs Insulin-like growth factors 胰岛素样生长因子
IL-1 Interleukin-1 白细胞介素-1
MAP2 Microtubule-assciated proterns2 微管相关蛋白 2
MCP-1 Monocyte chemoattractant protein1 单核细胞趋化蛋白
MTT Methylthio tetrazole 四甲基偶氮唑盐
NGF Nerve growth factor 神经生长因子
NT-4 Neurotrophic-4 神经营养因子 4
OD Optical density 光密度值
PAGE Polyacrylamidedel electrophoresis 聚丙烯酰胺
PBS Phosphate buffer solution 磷酸盐缓冲液
PLL Poly-L-Lysine 多聚赖氨酸
RGCs Retinal ganglion cells 视网膜神经节细胞
SDS Sodium dodecyl sulfate 十二烷基硫酸钠
TRITC Tetramethyl rhodaine isothiacyanate 四甲基异硫酸罗丹明
1
第三军医大学硕士学位论文
Effect of crystallines on survival and growth of rat
RGCs in vitro

Abstract

How to protect survival of RGCs and promote axonal regeneration after ON injury
has e a focus all over the world. Now, it has been proved that lens injury can promote
survival of RGCs and axonal regeneration. The induction of lens injury results in release of
lentogenic factors which promote survival of RGCs and stimulate axonal regeneration
throughout the optic pathway, and arrive at the thalamus at last. But, what’re on earth the
lentogenic factors? It has not been answered until today. Leon found that there were a lot of
macrophages in the retina after lens injury, and thought that activated macrophages played a
great role. However, there’s time discrepancy between macrophage infiltration and axonal
regeneration, and axonal regeneration can not be pletely by