文档介绍：254 食品与药品 Food and Drug 2010年第12卷第07期
高压脉冲电场法提取鹿筋蛋白质和浸膏的工艺研究
赵景辉,王再幸,王英平,赵伟刚,李荣峰,孙成贺
(中国农业科学院特产研究所,吉林吉林 132109)
摘要:目的考察快速提取鹿筋蛋白质和浸膏的最佳工艺。方法用正交试验法,以电场强度、脉冲数、料液比等3项因
素设计3个水平进行正交试验。结果鹿筋蛋白质最佳提取工艺为电场强度20 kV/cm,脉冲数8,料液比1:25。鹿筋浸膏最
佳提取工艺为电场强度16 kV/cm,脉冲数10,料液比1:25。结论此提取工艺可行,经济、省时、回收率高。
关键词:鹿筋;蛋白质;提取工艺;正交试验
中图分类号: 文献标识码: A 文章编号:1672-979X(2010)07-0254-03
Study on Extraction Technology of Deer Sinew by High Voltage Pulsed Electric Field
ZHAO Jing-hui, WANG Zai-xing, WANG Ying-ping, ZHAO Wei-gang, LI Rong-feng, SUN Cheng-he
(Institute of Special Wild Economic Animal and Plant Science CAAS, Jilin 132109, China)
Abstract: Objective To investigate a fast extraction technology of deer sinew to provide the reliable experimental
data and theoretical basis for the industrialized production. Methods The orthogonal test method was adopted for
study the effects of electric field intensity, pulse number, material to liquid ratio on protein extraction and extract yield
of deer sinew. Results The optimal extraction technology for deer sinew protein was as follows: electric field intensity
of 20 kV/cm, pulse number of 8, material to liquid ratio of 1:25. The optimal technology for deer sinew extract was
as follows: electric field intensity of 16 kV/cm, pulse number of 10, material to liquid ratio of 1:25. Conclusion The
extraction technology is feasible and economic, ti