文档介绍：RP-HPLC法测定人血浆中氢溴酸西酞普兰及其药动学
何海霞周远大杨辉
(重庆医科大学附属第一医院临床药理研究室, 重庆 400016)
[关键词] RP-HPLC法,氢溴酸西酞普兰, 血药浓度药动学
[摘要] 目的:建立RP-HPLC法测定血浆中氢溴酸西酞普兰(CTPR)。方法:采用Discovery® C18柱(5µm,150×),以心得安ZP)为内标,甲醇- CH3COONH4溶液-三乙胺(29∶21∶)为流动相,流速1 mL·min-1;在紫外240nm波长处检测。结果:CTPR血药浓度线性范围为 ~·mL—1(r=),·mL—1;高、中、%~%。结论:本法的灵敏度、准确度及回收率均能达到血浆药物浓度的测定要求,也可用于临床人体药代动力学的研究。
[中图分类号]
[文献标识码] A
[文章编号]
Determination of citalopram hydrobromide in Human Plasma by RP-HPLC
HE Hai-xia, ZHOU Yuan-da and Yang-hui
(Dept. of Clinical Pharmacology, The First Affiliated Hospital of Chongqing University of Medical Sciences, Chongqing 400016)
[Key words] RP-HPLC, citalopram hydrobromide (CTPR), drug concentration in plasma, ics;
[Abstract] AIM: To establish a RP-HPLC method for the determination of citalopram hydrobromide in human plasma and applied to determine drug concentration in plasma of healthy volunteers. METHODS: pounds were separated on a Discovery® C18 column (5μm,150mm×). Iinternal standard was diazepam. The mobile phase posed of acetonitrile-·L—1KH2PO4(29:21:), the flow rate was 1mL·min—1 . The detection wavelength was UV 240nm. RESULTS: A good linearity was obtained from ~·mL—1 in plasma with a correlation coefficient of . The lowest concentration detected was ·mL—1. The recovery of method w