文档介绍：
番茄中玉米素提取条件的优化#
夏润玺,高何瑞,张立军,曹慧颖,王可**
(沈阳农业大学生物科学技术学院)
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摘要:结合高效液相色谱(HPLC)研究了番茄中玉米素的提取纯化方法。玉米素对光不敏
感,可在 60℃下浓缩。玉米素固相萃取时,C18 小柱用 2ml 80%甲醇活化后再用 1ml pH=3.
0 的乙酸水活化效果较好;从番茄中提取玉米素时,C18 小柱洗脱剂使用 45%甲醇除杂效果
较好。准备进行 HPLC 的样品是否用流动相溶解对检测结果没有影响。本实验为研究玉米素
对番茄再生的调控提供了有效的提取方法。
关键词:玉米素,细胞分裂素,固相萃取,HPLC,番茄
中图分类号:请查阅《中国图书馆分类法》
Optimization the method used in zeatin purification from
tomato
XIA Runxi, GAO Herui, ZHANG Lijun, CAO Huiying, WANG Ke
(College of Biological Science and Biotechnology,Shenyang Agricultural University,Shenyang
110866)
Abstract: The study was conducted to establish the methods which to purify zeatin in tomato.
Zeatin was detected by high performance liquid chromatography (HPLC). Zeatin was insensitive
to light,and stable at 60℃. C18 SPE column would be washed sequentially with 2ml 80%
methanol,1ml water–acetic acid (pH=) before applying the zeatin to the column. 45% ethanol
would be used as eluent while purifying the zeatin from tomato. There was no effect to result if the
sample was dissolved with the mobile phase of HPLC. The paper gave the methods of purifying
zeatin for studies on regulation of tomato regeneration by zeatin.
Key words: zeatin,cytokinin,SPE,HPLC,Tomato
0 引言
激素对于调节植物的生长发育至关重要。在植物组织培养的实践中,通常也是利用外源
激素诱导外植体的再生。外源激素的作用机理可能是影响了内源激素的合成和分配[1],因
此了解植物再生过程中内源激素含量的动态变化,对于揭示激素调控植物再生的机理具有重
要意义。
番茄(Solanum lyco