中期因子在恶性胸腔积液诊断中的作用.doc

文档介绍：
中期因子在恶性胸腔积液诊断中的作用
许玉君,吕明明,侯亚义,王婷婷**
(南京大学医学院免疫学实验室,江苏省医学分子技术重点实验室,南京 210093)
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摘要:目的:研究中期因子(MidKine,MK)在恶性胸腔积液患者诊断中的价值。方法:收集
病人的胸腔积液,根据临床诊断结果分为恶性组和良性组,提取其中游离的的 RNA,利用
实时定量 PCR 检测 MK 的 mRNA 表达量。同时检测胸腔积液中的 CEA 和生化标志。结果:
恶性组的游离 MK mRNA ( vs , P<).MK 诊断的敏感性
和精确性达到 %和 %。CEA 和 MK 的联合诊断可以达到 %敏感性和 %精确
性。结论:检测胸腔积液中游离的 MK mRNA 具有较高的临床诊断精确性,有望作为一个
新的临床诊断手段。
关键词:胸腔积液;中期因子;诊断法;游离 mRNA
中图分类号:
Diagnostic role of cell-free midkine in malignant pleural
effusions
XU Yujun, LV Mingming, HOU Yayi, WANG Tingting
(Immunology and Reproduction Biology Lab,Medical School and State Key Laboratory of
Pharmaceutical Biotechology,Nanjing University, NanJing210093)
Abstract: Purpose: The aim of this study was to detect the cell-free mRNA expression of midkine
(MK) in patients with effusions and its diagnostic value. Methods: Effusions were collected
prospectively from patients. The cell-free RNA was extracted from effusions and the mRNA
expression of MK was detected using real-time PCR. The expression of CEA and biochemical
markers in effusions were also assayed.. Results: The expression of cell-free MK mRNA was
significantly higher in the malignant group than in the benign group ( vs , P<). The
sensitivity and diagnostic accuracy of MK was % and %, while bination of CEA
and MK reache