文档介绍：
Analysis of harides in the triterpene glycosides
from sea cucumbers by high performance liquid
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chromatographic (HPLC)#
GAO Xiaomeng, DONG Ping, XUE Changhu, XU Jie, WANG Yuming**
(College of Food Science and Technology, Ocean University of China, Qingdao 266003)
Abstract: To fast and effectively analyze the sugar chain of triterpene glycosides from sea cucumber, a
method was developed using a reverse-phase high-performance liquid chromatography (HPLC) with
1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. An optimized chromatographic condition was
established in the present study. The calibration curves of harides (D-glucose,
D-3-O-methylgucose, D-xylose and D-quinovose) were linear within the range of – 1 mmol/L
(R2 > ). The detection limits of these four harides were below 5 pmol. harides
of holothurin A and holothurin B were analyzed by this method. The result showed it was simple, rapid,
stable, and less sample consumed than previous methods.
Key
words:
sea cucumber; triterpene
glycoside; haride determination;
1-phenyl-3-methyl-5-pyrazolone (PMP); derivatization; high performance liquid chromatographic
(HPLC).
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0 Introduction
Much attention had been paid to triterpene glycosides isolated from sea cucumbers,
because they exhibit various biological activities, including antifungal, cytotoxic, hemolytic,